chrX : 74,259,954 – 74,260,568
614 bp 106 TFs 3 linked genes
This 614 bp open chromatin element is linked to FTX, ENSG00000271533, and SLC16A2 and is bound by 106 transcription factors.
Linked Genes
3 genes
Gene↕ Expression Dist. to TSS↑ Distance↕ Link type↕
FTX 33.4 kb Distal Multiome
ENSG00000271533 46.5 kb Distal Multiome
SLC16A2 161.3 kb Distal Multiome
Chromatin Accessibility

Genome Browser

Genomic view centred on this element. Tracks include ATAC-seq accessibility, RNA-seq expression, TF binding peaks, and element-to-gene loop arcs across the ESC → DE time course.

View limited to ±500 kb around this element.
For the full data, please see the UCSC track linked below.
chrX:74,254,954 – 74,265,568
This element Other elements Gene links ATAC-seq RNA-seq

Transcription factors with ChIP-seq peak overlap or TOBIAS motif footprint evidence at this element.

TF Binding
106 transcription factors
Source
Cell type
ARNT::HIF1A 3 datasets
Motif DE_12h DE_12h-ARNTHIF1A_MA0259.2 5 bp overlap
Motif DE_24h DE_24h-ARNTHIF1A_MA0259.2 5 bp overlap
Motif ES_0h ES_0h-ARNTHIF1A_MA0259.2 5 bp overlap
ATF2 2 datasets
ChIP H1 ENCFF295GZO 499 bp overlap
ChIP WA01 ENCSR000BQU.ATF2.WA01 136 bp overlap
Arid3a 1 dataset
Motif DE_12h DE_12h-Arid3a_MA0151.1 6 bp overlap
Arnt 3 datasets
Motif DE_12h DE_12h-Arnt_MA0004.1 6 bp overlap
Motif DE_24h DE_24h-Arnt_MA0004.1 6 bp overlap
Motif ES_0h ES_0h-Arnt_MA0004.1 6 bp overlap
BRD4 1 dataset
ChIP HEK293_sgNT GSE129407.BRD4.HEK293_sgNT 71 bp overlap
CLOCK 3 datasets
Motif DE_12h DE_12h-CLOCK_MA0819.3 7 bp overlap
Motif DE_24h DE_24h-CLOCK_MA0819.3 7 bp overlap
Motif ES_0h ES_0h-CLOCK_MA0819.3 7 bp overlap
Ddit3::Cebpa 2 datasets
Motif DE_12h DE_12h-Ddit3Cebpa_MA0019.2 10 bp overlap
Motif DE_24h DE_24h-Ddit3Cebpa_MA0019.2 10 bp overlap
FEZF2 3 datasets
Motif DE_12h DE_12h-FEZF2_MA2341.1 8 bp overlap
Motif DE_24h DE_24h-FEZF2_MA2341.1 8 bp overlap
Motif ES_0h ES_0h-FEZF2_MA2341.1 8 bp overlap
FOXP1 1 dataset
ChIP LNCaP GSE62492.FOXP1.LNCaP 111 bp overlap
Gli1 1 dataset
Motif DE_12h DE_12h-Gli1_MA1990.2 10 bp overlap
Gli2 1 dataset
Motif DE_12h DE_12h-Gli2_MA0734.4 9 bp overlap
HDAC2 1 dataset
ChIP H1 ENCFF939VKA 237 bp overlap
HIC2 1 dataset
Motif DE_12h DE_12h-HIC2_MA0738.2 6 bp overlap
IRF3 2 datasets
Motif DE_12h DE_12h-IRF3_MA1418.2 17 bp overlap
Motif DE_24h DE_24h-IRF3_MA1418.2 17 bp overlap
Isl1 1 dataset
Motif DE_12h DE_12h-Isl1_MA1608.2 7 bp overlap
JUN 1 dataset
ChIP ESC S34-ESC-d0-JUN-exp2 287 bp overlap
KLF13 3 datasets
Motif DE_12h DE_12h-KLF13_MA0657.2 17 bp overlap
Motif DE_24h DE_24h-KLF13_MA0657.2 17 bp overlap
Motif ES_0h ES_0h-KLF13_MA0657.2 17 bp overlap
KLF16 3 datasets
Motif DE_12h DE_12h-KLF16_MA0741.1 11 bp overlap
Motif DE_24h DE_24h-KLF16_MA0741.1 11 bp overlap
Motif ES_0h ES_0h-KLF16_MA0741.1 11 bp overlap
KLF5 2 datasets
Motif DE_12h DE_12h-KLF5_MA0599.1 10 bp overlap
Motif DE_24h DE_24h-KLF5_MA0599.1 10 bp overlap
KLF9 3 datasets
Motif DE_12h DE_12h-KLF9_MA1107.3 11 bp overlap
Motif DE_24h DE_24h-KLF9_MA1107.3 11 bp overlap
Motif ES_0h ES_0h-KLF9_MA1107.3 11 bp overlap
MAX 5 datasets
Motif DE_12h DE_12h-MAX_MA0058.4 6 bp overlap
Motif DE_24h DE_24h-MAX_MA0058.4 6 bp overlap
Motif ES_0h ES_0h-MAX_MA0058.4 6 bp overlap
ChIP H1 ENCFF914VQY 357 bp overlap
ChIP WA01 ENCSR000EUP.MAX.WA01 230 bp overlap
MAZ 2 datasets
Motif DE_12h DE_12h-MAZ_MA1522.2 8 bp overlap
Motif DE_24h DE_24h-MAZ_MA1522.2 8 bp overlap
MNT 3 datasets
Motif DE_12h DE_12h-MNT_MA0825.2 6 bp overlap
Motif DE_24h DE_24h-MNT_MA0825.2 6 bp overlap
Motif ES_0h ES_0h-MNT_MA0825.2 6 bp overlap
MSC 2 datasets
Motif DE_12h DE_12h-MSC_MA0665.1 10 bp overlap
Motif DE_24h DE_24h-MSC_MA0665.1 10 bp overlap
MXI1 2 datasets
Motif DE_12h DE_12h-MXI1_MA1108.3 6 bp overlap
Motif DE_24h DE_24h-MXI1_MA1108.3 6 bp overlap
MYC 5 datasets
Motif DE_12h DE_12h-MYC_MA0147.4 8 bp overlap
Motif DE_24h DE_24h-MYC_MA0147.4 8 bp overlap
Motif ES_0h ES_0h-MYC_MA0147.4 8 bp overlap
ChIP H1 ENCFF794ZJT 265 bp overlap
ChIP WA01 ENCSR000EBY.MYC.WA01 119 bp overlap
MYCN 3 datasets
Motif DE_12h DE_12h-MYCN_MA0104.5 8 bp overlap
Motif DE_24h DE_24h-MYCN_MA0104.5 8 bp overlap
Motif ES_0h ES_0h-MYCN_MA0104.5 8 bp overlap
MZF1 5 datasets
Motif DE_12h DE_12h-MZF1_MA0056.3 8 bp overlap
Motif DE_12h DE_12h-MZF1_MA0056.3 8 bp overlap
Motif DE_24h DE_24h-MZF1_MA0056.3 8 bp overlap
Motif DE_24h DE_24h-MZF1_MA0056.3 8 bp overlap
Motif ES_0h ES_0h-MZF1_MA0056.3 8 bp overlap
Mlxip 3 datasets
Motif DE_12h DE_12h-Mlxip_MA0622.2 6 bp overlap
Motif DE_24h DE_24h-Mlxip_MA0622.2 6 bp overlap
Motif ES_0h ES_0h-Mlxip_MA0622.2 6 bp overlap
NANOG 2 datasets
ChIP HUES-8 GSE109524.NANOG.HUES-8 278 bp overlap
ChIP WA01 ENCSR000BMT.NANOG.WA01 147 bp overlap
NFATC3 2 datasets
Motif DE_12h DE_12h-NFATC3_MA0625.3 6 bp overlap
Motif DE_24h DE_24h-NFATC3_MA0625.3 6 bp overlap
NFATC4 2 datasets
Motif DE_12h DE_12h-NFATC4_MA1525.3 9 bp overlap
Motif DE_24h DE_24h-NFATC4_MA1525.3 9 bp overlap
NFIB 2 datasets
Motif DE_12h DE_12h-NFIB_MA1643.2 17 bp overlap
Motif DE_24h DE_24h-NFIB_MA1643.2 17 bp overlap
NR4A1 2 datasets
Motif DE_12h DE_12h-NR4A1_MA1112.3 8 bp overlap
Motif DE_24h DE_24h-NR4A1_MA1112.3 8 bp overlap
NR4A2 2 datasets
Motif DE_12h DE_12h-NR4A2_MA0160.3 8 bp overlap
Motif DE_24h DE_24h-NR4A2_MA0160.3 8 bp overlap
Nfat5 2 datasets
Motif DE_12h DE_12h-Nfat5_MA0606.3 8 bp overlap
Motif DE_24h DE_24h-Nfat5_MA0606.3 8 bp overlap
Nfatc1 2 datasets
Motif DE_12h DE_12h-Nfatc1_MA0624.3 6 bp overlap
Motif DE_24h DE_24h-Nfatc1_MA0624.3 6 bp overlap
Nfatc2 2 datasets
Motif DE_12h DE_12h-Nfatc2_MA0152.3 8 bp overlap
Motif DE_24h DE_24h-Nfatc2_MA0152.3 8 bp overlap
Npas2 3 datasets
Motif DE_12h DE_12h-Npas2_MA0626.2 8 bp overlap
Motif DE_24h DE_24h-Npas2_MA0626.2 8 bp overlap
Motif ES_0h ES_0h-Npas2_MA0626.2 8 bp overlap
PATZ1 2 datasets
Motif DE_12h DE_12h-PATZ1_MA1961.2 11 bp overlap
Motif DE_24h DE_24h-PATZ1_MA1961.2 11 bp overlap
POU1F1 4 datasets
Motif DE_12h DE_12h-POU1F1_MA0784.3 14 bp overlap
Motif DE_12h DE_12h-POU1F1_MA0784.3 14 bp overlap
Motif DE_12h DE_12h-POU1F1_MA0784.3 14 bp overlap
Motif DE_24h DE_24h-POU1F1_MA0784.3 14 bp overlap
POU2F1 4 datasets
Motif DE_12h DE_12h-POU2F1_MA0785.2 9 bp overlap
Motif DE_12h DE_12h-POU2F1_MA0785.2 9 bp overlap
Motif DE_12h DE_12h-POU2F1_MA0785.2 9 bp overlap
Motif DE_24h DE_24h-POU2F1_MA0785.2 9 bp overlap
POU2F1::SOX2 2 datasets
Motif DE_12h DE_12h-POU2F1SOX2_MA1962.1 17 bp overlap
Motif DE_12h DE_12h-POU2F1SOX2_MA1962.1 17 bp overlap
POU2F2 4 datasets
Motif DE_12h DE_12h-POU2F2_MA0507.3 13 bp overlap
Motif DE_12h DE_12h-POU2F2_MA0507.3 13 bp overlap
Motif DE_12h DE_12h-POU2F2_MA0507.3 13 bp overlap
Motif DE_24h DE_24h-POU2F2_MA0507.3 13 bp overlap
POU2F3 4 datasets
Motif DE_12h DE_12h-POU2F3_MA0627.3 9 bp overlap
Motif DE_12h DE_12h-POU2F3_MA0627.3 9 bp overlap
Motif DE_12h DE_12h-POU2F3_MA0627.3 9 bp overlap
Motif DE_24h DE_24h-POU2F3_MA0627.3 9 bp overlap
POU3F1 4 datasets
Motif DE_12h DE_12h-POU3F1_MA0786.2 10 bp overlap
Motif DE_12h DE_12h-POU3F1_MA0786.2 10 bp overlap
Motif DE_12h DE_12h-POU3F1_MA0786.2 10 bp overlap
Motif DE_24h DE_24h-POU3F1_MA0786.2 10 bp overlap
POU3F2 4 datasets
Motif DE_12h DE_12h-POU3F2_MA0787.1 12 bp overlap
Motif DE_12h DE_12h-POU3F2_MA0787.1 12 bp overlap
Motif DE_12h DE_12h-POU3F2_MA0787.1 12 bp overlap
Motif DE_24h DE_24h-POU3F2_MA0787.1 12 bp overlap
POU3F3 4 datasets
Motif DE_12h DE_12h-POU3F3_MA0788.1 13 bp overlap
Motif DE_12h DE_12h-POU3F3_MA0788.1 13 bp overlap
Motif DE_12h DE_12h-POU3F3_MA0788.1 13 bp overlap
Motif DE_24h DE_24h-POU3F3_MA0788.1 13 bp overlap
POU3F4 4 datasets
Motif DE_12h DE_12h-POU3F4_MA0789.1 9 bp overlap
Motif DE_12h DE_12h-POU3F4_MA0789.1 9 bp overlap
Motif DE_12h DE_12h-POU3F4_MA0789.1 9 bp overlap
Motif DE_24h DE_24h-POU3F4_MA0789.1 9 bp overlap
POU4F1 1 dataset
Motif DE_12h DE_12h-POU4F1_MA0790.2 12 bp overlap
POU4F2 1 dataset
Motif DE_12h DE_12h-POU4F2_MA0683.2 15 bp overlap
POU4F3 1 dataset
Motif DE_12h DE_12h-POU4F3_MA0791.2 12 bp overlap
POU5F1 11 datasets
ChIP BG03 GSE21614.POU5F1.BG03 153 bp overlap
Motif DE_12h DE_12h-POU5F1_MA1115.2 7 bp overlap
Motif DE_12h DE_12h-POU5F1_MA1115.2 7 bp overlap
Motif DE_12h DE_12h-POU5F1_MA1115.2 7 bp overlap
Motif DE_24h DE_24h-POU5F1_MA1115.2 7 bp overlap
ChIP DE_D1 DED1-OCT4_Batch_II 404 bp overlap
ChIP GM23338 ENCFF333SNB 225 bp overlap
ChIP H1 ENCFF698ZAP 143 bp overlap
ChIP HUES-8 GSE109524.POU5F1.HUES-8 290 bp overlap
ChIP hESC_NAIVE GSE69646.POU5F1.hESC_NAIVE 614 bp overlap
ChIP hESC_PRIMED GSE69646.POU5F1.hESC_PRIMED 481 bp overlap
POU5F1B 4 datasets
Motif DE_12h DE_12h-POU5F1B_MA0792.1 9 bp overlap
Motif DE_12h DE_12h-POU5F1B_MA0792.1 9 bp overlap
Motif DE_12h DE_12h-POU5F1B_MA0792.1 9 bp overlap
Motif DE_24h DE_24h-POU5F1B_MA0792.1 9 bp overlap
POU5F1_M 1 dataset
ChIP DE_D1 DED1-OCT4-M_Batch_II 303 bp overlap
PRDM9 2 datasets
Motif DE_12h DE_12h-PRDM9_MA1723.2 20 bp overlap
Motif DE_24h DE_24h-PRDM9_MA1723.2 20 bp overlap
Pou5f1::Sox2 4 datasets
Motif DE_12h DE_12h-Pou5f1Sox2_MA0142.1 15 bp overlap
Motif DE_12h DE_12h-Pou5f1Sox2_MA0142.1 15 bp overlap
Motif DE_12h DE_12h-Pou5f1Sox2_MA0142.1 15 bp overlap
Motif DE_24h DE_24h-Pou5f1Sox2_MA0142.1 15 bp overlap
Pparg::Rxra 2 datasets
Motif DE_12h DE_12h-PpargRxra_MA0065.3 13 bp overlap
Motif DE_24h DE_24h-PpargRxra_MA0065.3 13 bp overlap
Prdm14 2 datasets
Motif DE_12h DE_12h-Prdm14_MA1998.2 8 bp overlap
Motif DE_24h DE_24h-Prdm14_MA1998.2 8 bp overlap
Prdm15 2 datasets
Motif DE_12h DE_12h-Prdm15_MA1616.2 11 bp overlap
Motif DE_24h DE_24h-Prdm15_MA1616.2 11 bp overlap
Prdm4 1 dataset
Motif DE_12h DE_12h-Prdm4_MA1647.3 11 bp overlap
RFX4 2 datasets
Motif DE_12h DE_12h-RFX4_MA0799.3 13 bp overlap
Motif DE_24h DE_24h-RFX4_MA0799.3 13 bp overlap
Rfx6 2 datasets
Motif DE_12h DE_12h-Rfx6_MA1724.2 9 bp overlap
Motif DE_24h DE_24h-Rfx6_MA1724.2 9 bp overlap
SCRT1 2 datasets
Motif DE_12h DE_12h-SCRT1_MA0743.3 10 bp overlap
Motif DE_24h DE_24h-SCRT1_MA0743.3 10 bp overlap
SOX10 2 datasets
Motif DE_12h DE_12h-SOX10_MA0442.3 6 bp overlap
Motif DE_24h DE_24h-SOX10_MA0442.3 6 bp overlap
SOX2 2 datasets
ChIP hESC GSE69479.SOX2.hESC 180 bp overlap
ChIP hiPSC GSE56567.SOX2.hiPSC 192 bp overlap
SOX9 1 dataset
Motif DE_12h DE_12h-SOX9_MA0077.2 8 bp overlap
SP4 2 datasets
Motif DE_12h DE_12h-SP4_MA0685.2 9 bp overlap
Motif DE_24h DE_24h-SP4_MA0685.2 9 bp overlap
SP5 4 datasets
Motif DE_12h DE_12h-SP5_MA1965.2 6 bp overlap
Motif DE_12h DE_12h-SP5_MA1965.2 6 bp overlap
Motif DE_24h DE_24h-SP5_MA1965.2 6 bp overlap
Motif DE_24h DE_24h-SP5_MA1965.2 6 bp overlap
SP8 3 datasets
Motif DE_12h DE_12h-SP8_MA0747.2 11 bp overlap
Motif DE_24h DE_24h-SP8_MA0747.2 11 bp overlap
Motif ES_0h ES_0h-SP8_MA0747.2 11 bp overlap
SP9 3 datasets
Motif DE_12h DE_12h-SP9_MA1564.2 10 bp overlap
Motif DE_24h DE_24h-SP9_MA1564.2 10 bp overlap
Motif ES_0h ES_0h-SP9_MA1564.2 10 bp overlap
SPI1 1 dataset
ChIP K-562 GSE70482.SPI1.K-562 172 bp overlap
SPIC 2 datasets
Motif DE_12h DE_12h-SPIC_MA0687.2 13 bp overlap
Motif DE_24h DE_24h-SPIC_MA0687.2 13 bp overlap
SRF 2 datasets
ChIP H1 ENCFF036PEF 183 bp overlap
ChIP WA01 ENCSR000BIV.SRF.WA01 136 bp overlap
STAT1 2 datasets
Motif DE_12h DE_12h-STAT1_MA0137.4 9 bp overlap
Motif DE_24h DE_24h-STAT1_MA0137.4 9 bp overlap
STAT3 2 datasets
Motif DE_12h DE_12h-STAT3_MA0144.3 9 bp overlap
Motif DE_24h DE_24h-STAT3_MA0144.3 9 bp overlap
Spi1 2 datasets
Motif DE_12h DE_12h-Spi1_MA0080.7 13 bp overlap
Motif DE_24h DE_24h-Spi1_MA0080.7 13 bp overlap
Stat4 2 datasets
Motif DE_12h DE_12h-Stat4_MA0518.2 10 bp overlap
Motif DE_24h DE_24h-Stat4_MA0518.2 10 bp overlap
TBP 2 datasets
ChIP hESC GSE122298.TBP.hESC 140 bp overlap
ChIP hESC_2h GSE122298.TBP.hESC_2h 215 bp overlap
TFAP2A 1 dataset
Motif DE_12h DE_12h-TFAP2A_MA0810.2 11 bp overlap
TFAP2B 1 dataset
Motif DE_12h DE_12h-TFAP2B_MA0811.2 11 bp overlap
TFAP2C 1 dataset
Motif DE_12h DE_12h-TFAP2C_MA0524.3 11 bp overlap
TFAP2E 1 dataset
Motif DE_12h DE_12h-TFAP2E_MA1569.2 9 bp overlap
TRIM28 1 dataset
ChIP WIBR3_NAIVE GSE84382.TRIM28.WIBR3_NAIVE 95 bp overlap
USF1 4 datasets
Motif DE_12h DE_12h-USF1_MA0093.4 10 bp overlap
Motif DE_24h DE_24h-USF1_MA0093.4 10 bp overlap
ChIP H1 ENCFF090WVU 241 bp overlap
ChIP WA01 ENCSR000BIU.USF1.WA01 206 bp overlap
VEZF1 2 datasets
Motif DE_12h DE_12h-VEZF1_MA1578.2 6 bp overlap
Motif DE_24h DE_24h-VEZF1_MA1578.2 6 bp overlap
Wt1 5 datasets
Motif DE_12h DE_12h-Wt1_MA1627.2 10 bp overlap
Motif DE_12h DE_12h-Wt1_MA1627.2 10 bp overlap
Motif DE_24h DE_24h-Wt1_MA1627.2 10 bp overlap
Motif DE_24h DE_24h-Wt1_MA1627.2 10 bp overlap
Motif ES_0h ES_0h-Wt1_MA1627.2 10 bp overlap
YY1 4 datasets
ChIP H1 ENCFF524BTL 164 bp overlap
ChIP NT2-D1 ENCSR000EXG.YY1.NT2-D1 157 bp overlap
ChIP NT2/D1 ENCFF999MII 309 bp overlap
ChIP WA01 ENCSR000BKD.YY1.WA01 288 bp overlap
Yy1 2 datasets
Motif DE_12h DE_12h-Yy1_MA0095.4 8 bp overlap
Motif DE_24h DE_24h-Yy1_MA0095.4 8 bp overlap
ZBTB6 2 datasets
Motif DE_12h DE_12h-ZBTB6_MA1581.2 9 bp overlap
Motif DE_24h DE_24h-ZBTB6_MA1581.2 9 bp overlap
ZFP42 2 datasets
Motif DE_12h DE_12h-ZFP42_MA1651.2 13 bp overlap
Motif DE_24h DE_24h-ZFP42_MA1651.2 13 bp overlap
ZNF148 2 datasets
Motif DE_12h DE_12h-ZNF148_MA1653.2 10 bp overlap
Motif DE_24h DE_24h-ZNF148_MA1653.2 10 bp overlap
ZNF211 3 datasets
Motif DE_12h DE_12h-ZNF211_MA1974.2 10 bp overlap
Motif DE_24h DE_24h-ZNF211_MA1974.2 10 bp overlap
Motif ES_0h ES_0h-ZNF211_MA1974.2 10 bp overlap
ZNF281 2 datasets
Motif DE_12h DE_12h-ZNF281_MA1630.3 10 bp overlap
Motif DE_24h DE_24h-ZNF281_MA1630.3 10 bp overlap
ZNF320 2 datasets
Motif DE_12h DE_12h-ZNF320_MA1976.2 20 bp overlap
Motif DE_24h DE_24h-ZNF320_MA1976.2 20 bp overlap
ZNF530 2 datasets
Motif DE_12h DE_12h-ZNF530_MA1981.2 14 bp overlap
Motif DE_24h DE_24h-ZNF530_MA1981.2 14 bp overlap
ZNF547 1 dataset
Motif DE_12h DE_12h-ZNF547_MA2334.1 13 bp overlap
ZNF675 2 datasets
Motif DE_12h DE_12h-ZNF675_MA1714.2 19 bp overlap
Motif DE_24h DE_24h-ZNF675_MA1714.2 19 bp overlap
ZNF692 1 dataset
Motif DE_12h DE_12h-ZNF692_MA1986.2 8 bp overlap
ZNF740 3 datasets
Motif DE_12h DE_12h-ZNF740_MA0753.3 10 bp overlap
Motif DE_24h DE_24h-ZNF740_MA0753.3 10 bp overlap
Motif ES_0h ES_0h-ZNF740_MA0753.3 10 bp overlap
ZNF75A 2 datasets
Motif DE_12h DE_12h-ZNF75A_MA2097.1 12 bp overlap
Motif DE_24h DE_24h-ZNF75A_MA2097.1 12 bp overlap
ZNF816 3 datasets
Motif DE_12h DE_12h-ZNF816_MA1719.2 15 bp overlap
Motif DE_24h DE_24h-ZNF816_MA1719.2 15 bp overlap
Motif ES_0h ES_0h-ZNF816_MA1719.2 15 bp overlap
ZSCAN29 3 datasets
Motif DE_12h DE_12h-ZSCAN29_MA1602.2 11 bp overlap
Motif ES_0h ES_0h-ZSCAN29_MA1602.2 11 bp overlap
ChIP K-562 ENCSR175SZH.ZSCAN29.K-562 146 bp overlap
Zfp961 2 datasets
Motif DE_12h DE_12h-Zfp961_MA2126.1 8 bp overlap
Motif ES_0h ES_0h-Zfp961_MA2126.1 8 bp overlap